FITC-Dextran (MW 4000) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (4 kDa). FITC-Dextran are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used^[1][2][3].

4000.00(Average)

60842-46-8

熒光素異硫氰酸酯-葡聚糖(MW 4000)

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DMSO : 16.67 mg/mL (Need ultrasonic)

H₂O : 16.67 mg/mL (Need ultrasonic)

請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨?。以下溶解方案都請先按?In Vitro 方式配制澄清的儲備液,，再依次添加助溶劑：

——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件,，適當保存,；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配,，當天使用,； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀,、析出現(xiàn)象,，可以通過加熱和/或超聲的方式助溶

• 1.

  請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: 1.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic

• 2.

  請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: 1.67 mg/mL (Infinity mM); Suspended solution; Need ultrasonic

• 3.

  請依序添加每種溶劑： 10% DMSO    90% corn oil

  Solubility: 1.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic

• [1]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.

  [2]. Hoffmann A, et al. High and Low Molecular Weight Fluorescein Isothiocyanate (FITC)-Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations. Transl Stroke Res. 2011 Mar;2(1):106-11.

  [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.