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本公眾號每天分享一篇最新一期Anesthesia & Analgesia等SCI雜志的摘要翻譯,，敬請關注并提出寶貴意見  Sevoflurane preconditioning ameliorates lipopolysaccharide-induced cognitive impairment in mice  背景與目的：全身性炎癥誘發(fā)腦神經細胞炎癥，進而引起急性認知功能障礙,。此外,，神經元炎癥是術后認知功能障礙和譫妄的一個原因,。然而，沒有合適的藥物可用于治療神經認知性皮炎,。本研究評估了用七氟烷麻醉預處理對脂多糖（LPS）誘導的系統(tǒng)性炎癥的動物模型中的認知和神經炎癥變化導致的可能的神經保護作用,。  1方法：成年小鼠隨機分為（1）對照組，（2）2%七氟烷預處理1h, (3)腹腔注射LPS 5mg/kg,，(4)2﹪七氟烷預處理1h+LPS注射組,。再注射5mg/kg LPS后24小時，使用免疫染色和免疫印跡測定海馬中小膠質細胞標記物離子鈣接頭蛋白抗體(Iba-1),。采用1L-1β和1L-6作為免疫印跡法的炎癥標記物,，并進行淀粉樣蛋白前體蛋白裂解酶1（BACE1）免疫印跡的β位以評估淀粉樣蛋白β-蛋白（Αβ）積聚。長期認知功能障礙評估使用恐懼條件測試,。 結果：腹腔內LPS增加了Iba-1（150％）,，炎癥標志物（160％）和Αβ積聚物（350％）的水平，并且七氟烷預處理抑制了這些增加,。全身LPS導致學習障礙,。七氟烷還維持接受LPS注射小鼠的長期記憶。七氟烷預處理通過減少過度小膠質細胞活化,，炎癥和Αβ積聚來預防全身LPS小鼠模型所致的長期記憶障礙,。 結論：本研究支持七氟烷預處理可能對神經元炎癥有益的假說。七氟烷可能對減少術后認知功能障礙和譫妄有益,。 Maiko Satomoto1,2)*, Zhongliang Sun1)*, Yushi U. Adachi1),Hiroyuki Kinoshita3), and Koshi Makita1) Sevoflurane preconditioning ameliorates lipopolysaccharide-induced cognitive impairment in mice. BACKGROUND:Systemic inflammation induces brain neuronal inflammation, in turn causing acute cognitive disorders. Furthermore, neuronal inflammation is one cause of postoperative cognitive disorder (POCD) and delirium. However, no sufficiently established pharmacological treatment is available for neurocognitive inflammation. This study evaluated the possible neuroprotective effects of preconditioning with sevoflurane anesthesia on cognition and neuroinflammatory changes in an animal model of lipopolysaccharide (LPS)-induced systemic inflammation METHODS:Adult mice were randomly divided into (1) control, (2) 2% sevoflurane preconditioning for 1 h, (3) intraperitoneal 5 mg/kg LPS injection, and (4) 2% sevoflurane preconditioning for 1 h + LPS injection groups. At 24 h after 5 mg/kg LPS injection, microglial activation based on ionized calcium-binding adapter molecule 1 (Iba-1) expression in the hippocampus was determined using immunostaining and immunoblotting. IL-1β and IL-6 immunoblotting were used as inflammation markers, and β-site of amyloid precursor protein cleaving enzyme 1 (BACE1) immunoblotting was performed to evaluate amyloid β-protein (Aβ) accumulation. Long-term cognitive impairment was evaluated using fear conditioning tests. RESULTS:Intraperitoneal LPS increased levels of Iba-1 (150%), inflammation markers (160%), and Aβ accumulation (350%), and sevoflurane preconditioning suppressed these increases. Systemic LPS caused learning deficits. Sevoflurane also maintained long-term memory in mice receiving LPS injection. Sevoflurane preconditioning prevented long-term memory impairment in the mouse model administered systemic LPS by decreasing excessive microglial activation, inflammation, and Aβ accumulation. CONCLUSIONS:This study supports the hypothesis that sevoflurane preconditioning might also be beneficial for neuronal inflammation. Sevoflurane might be beneficial for reducing delirium and POCD. 麻醉學文獻進展分享 聯(lián)系我們 |
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